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Amersham Life Sciences Inc hiload 16 60 superdex 75 column
Hiload 16 60 Superdex 75 Column, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hiload+16+60+superdex+75+column/16+60+75+column+hiload+superdex/pm18989959__ja806779e_si_001-123-13-18
Average 86 stars, based on 1 article reviews
hiload 16 60 superdex 75 column - by Bioz Stars, 2026-10
86/100 stars

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Purification:

Article Title: Site-specific N- and C-terminal labeling of a single polypeptide using sortases of different specificity
Article Snippet: This material was then purified by anion-exchange chromatography on a Mono Q 5/50 GL column (Amersham) [Buffer A (50 mM Tris pH 7.5, 5 mM DTT, 0.5 mM EDTA), Buffer B (50 mM Tris pH 7.5, 5 mM DTT, 0.5 mM EDTA, 500 mM NaCl), 1.5 mL/min, gradient: 100% Buffer A (0-15 mL), 0% Buffer B → 50% Buffer B (15-45 mL), 50% Buffer B (45-50 mL)]. .. Fractions containing UCHL3 were pooled and further purified by size exclusion chromatography on a HiLoad 16/60 Superdex 75 column (Amersham), eluting with 20 mM Tris pH 8.0, 150 mM NaCl at a flow rate of 1 mL/min. .. Fractions containing UCHL3 were pooled and subjected to a final purification step by anion-exchange chromatography on a Mono Q 5/50 GL column (Amersham) [Buffer A (50 mM phosphate pH 6.0), Buffer B (50 mM phosphate pH 6.0, 500 mM NaCl), 1.5 mL/min, gradient: 100% Buffer A (0-15 mL), 0% Buffer B → 50% Buffer B (15-45 mL), 50% Buffer B (45-50 mL)].

Article Title: Enzymes as Tools for Building and Manipulating Nanomaterials /
Article Snippet: .. Fractions containing Sortase A were pooled and further purified by size exclusion chromatography on a HiLoad 16/60 Superdex 75 column (Amersham), eluting with 20 mM Tris pH 8.0, 150 mM NaCl at a flow rate of 2 mL/min. ..

Article Title: Supporting Information 1 Lipid Modification of Proteins through Sortase-Catalyzed Transpeptidation
Article Snippet: .. This material was concentrated and further purified by size exclusion chromatography on a HiLoad 16/60 Superdex 75 column (Amersham), eluting with 20 mM Tris pH 8.0, 150 mM NaCl at a flow rate of 1 mL/min. ..

Article Title: Site-specific N- and C-terminal labeling of a single polypeptide using sortases of different specificity
Article Snippet: .. This material was concentrated and further purified by size exclusion chromatography on a HiLoad 16/60 Superdex 75 column (Amersham), eluting with 20 mM Tris pH 8.0, 150 mM NaCl at a flow rate of 1 mL/min. ..

Article Title: Site-specific N- and C-terminal labeling of a single polypeptide using sortases of different specificity
Article Snippet: .. Fractions containing SrtAstrep were pooled and further purified by size exclusion chromatography on a HiLoad 16/60 Superdex 75 column (Amersham), eluting with 20 mM Tris pH 8.0, 150 mM NaCl at a flow rate of 1 mL/min. ..

Size-exclusion Chromatography:

Article Title: Site-specific N- and C-terminal labeling of a single polypeptide using sortases of different specificity
Article Snippet: This material was then purified by anion-exchange chromatography on a Mono Q 5/50 GL column (Amersham) [Buffer A (50 mM Tris pH 7.5, 5 mM DTT, 0.5 mM EDTA), Buffer B (50 mM Tris pH 7.5, 5 mM DTT, 0.5 mM EDTA, 500 mM NaCl), 1.5 mL/min, gradient: 100% Buffer A (0-15 mL), 0% Buffer B → 50% Buffer B (15-45 mL), 50% Buffer B (45-50 mL)]. .. Fractions containing UCHL3 were pooled and further purified by size exclusion chromatography on a HiLoad 16/60 Superdex 75 column (Amersham), eluting with 20 mM Tris pH 8.0, 150 mM NaCl at a flow rate of 1 mL/min. .. Fractions containing UCHL3 were pooled and subjected to a final purification step by anion-exchange chromatography on a Mono Q 5/50 GL column (Amersham) [Buffer A (50 mM phosphate pH 6.0), Buffer B (50 mM phosphate pH 6.0, 500 mM NaCl), 1.5 mL/min, gradient: 100% Buffer A (0-15 mL), 0% Buffer B → 50% Buffer B (15-45 mL), 50% Buffer B (45-50 mL)].

Article Title: Enzymes as Tools for Building and Manipulating Nanomaterials /
Article Snippet: .. Fractions containing Sortase A were pooled and further purified by size exclusion chromatography on a HiLoad 16/60 Superdex 75 column (Amersham), eluting with 20 mM Tris pH 8.0, 150 mM NaCl at a flow rate of 2 mL/min. ..

Article Title: Supporting Information 1 Lipid Modification of Proteins through Sortase-Catalyzed Transpeptidation
Article Snippet: .. This material was concentrated and further purified by size exclusion chromatography on a HiLoad 16/60 Superdex 75 column (Amersham), eluting with 20 mM Tris pH 8.0, 150 mM NaCl at a flow rate of 1 mL/min. ..

Article Title: Site-specific N- and C-terminal labeling of a single polypeptide using sortases of different specificity
Article Snippet: .. This material was concentrated and further purified by size exclusion chromatography on a HiLoad 16/60 Superdex 75 column (Amersham), eluting with 20 mM Tris pH 8.0, 150 mM NaCl at a flow rate of 1 mL/min. ..

Article Title: Supporting Information 1 Lipid Modification of Proteins through Sortase-Catalyzed Transpeptidation
Article Snippet: HPLC purifications were achieved using an Agilent 1100 Series HPLC system equipped with a Waters Delta Pak 5 μm, 100 Å C4 column (3.9 x 150 mm, Supporting Information 3 MeCN:H2O gradient mobile phase containing 0.1% trifluoroacetic acid, 1 mL/min), a Waters Delta Pak 15 μm, 100 Å C18 column (7.8 x 300 mm, 2-propanol:H2O gradient mobile phase, 2 mL/min), or a Waters Cosmosil 5PE column (8 x 250 mm, 2- propanol:H2O gradient mobile phase, 1 mL/min) as indicated below. .. Size exclusion chromatography was performed on a Pharmacia AKTA Purifier system equipped with a HiLoad 16/60 Superdex 75 column (Amersham). ..

Article Title: Site-specific N- and C-terminal labeling of a single polypeptide using sortases of different specificity
Article Snippet: .. Fractions containing SrtAstrep were pooled and further purified by size exclusion chromatography on a HiLoad 16/60 Superdex 75 column (Amersham), eluting with 20 mM Tris pH 8.0, 150 mM NaCl at a flow rate of 1 mL/min. ..

Chromatography:

Article Title: Site-specific N- and C-terminal labeling of a single polypeptide using sortases of different specificity
Article Snippet: HPLC purifications were achieved using an Agilent 1100 Series HPLC system equipped with a Waters Delta Pak 15 μm, 100 Å C18 column (7.8 x 300 mm, MeCN:H2O gradient mobile phase, 3 mL/min) as indicated below. .. Size exclusion and cation exchange chromatography were performed on a Pharmacia AKTA Purifier system equipped with a HiLoad 16/60 Superdex 75 column (Amersham) or a Mono Q 5/50 GL column (Amersham), respectively. ..



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